So I've been trying to run Padena but it keeps finding ambiguous DNA so I grabbed one to see what it was finding and the letters it's saying that are in the alphabet clearly aren't. Does anyone know why this would happen?
As you can see below the actual sequence doesn't have what the Alphabet says it does.
This is from the Immediate Window:
reads.First().ConvertToString(0,reads.First().Count) //my first sequence from the "reads" collection